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(Mouse) Dpf2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC - (Mouse) Dpf2 Antibody (N-term) AW5514-U200
    AW5514 staining (Mouse) Dpf2 in mouse testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC - (Mouse) Dpf2 Antibody (N-term) AW5514-U200
    AW5514 staining (Mouse) Dpf2 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - (Mouse) Dpf2 Antibody (N-term) AW5514-U200
    All lanes : Anti-Dpf2 Antibody (N-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: mouse thymus lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q61103
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW M=44;H=44,24 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 19708
Antigen Region 99-133 aa
Other Names Zinc finger protein ubi-d4, Apoptosis response zinc finger protein, BRG1-associated factor 45D, BAF45D, D4, zinc and double PHD fingers family 2, Protein requiem, Dpf2, Baf45d, Req, Ubid4
Dilution IHC~~1:25
WB~~1:2000
Target/Specificity This mouse Dpf2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 99-133 amino acids from the N-terminal region of mouse Dpf2.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Dpf2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Dpf2
Synonyms Baf45d, Req, Ubid4
Function May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Cellular Location Cytoplasm. Nucleus.
Tissue Location In embryo, highest levels are seen in brain, eyes, thymus and olfactory epithelium in nose, whereas several other tissues, including the musculoskeletal system, show moderate expression. In adult, higher expression in testis, medium in thymus and spleen, lower in certain parts of the brain as the hippocampus. No expression in adult heart, lung, liver, duodenum and kidney.
Research Areas
Citations (0)

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Background

May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.

References

Mertsalov I.B.,et al.Mamm. Genome 11:72-74(2000).
Carninci P.,et al.Science 309:1559-1563(2005).
Gabig T.G.,et al.J. Biol. Chem. 269:29515-29519(1994).
Gabig T.G.,et al.Mamm. Genome 9:660-665(1998).
Lessard J.,et al.Neuron 55:201-215(2007).

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$ 325.00
$ 110.00
Cat# AW5514-U200
Size:
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(40 western blots)
Availability: In Stock
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