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ACAT1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ACAT1 Antibody (C-term) AW5517-U100
    All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: mouse liver lysates Lane 3: SK-BR-3 whole cell lysates Lane 4: SW620 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - ACAT1 Antibody (C-term) AW5517-U100
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat.#AW5517), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB
Primary Accession P24752
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=45.2 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 38
Antigen Region 311-349 aa
Other Names Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT
Dilution WB~~1:1000
IHC-P~~1:10~50
Target/Specificity This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-349 amino acids from the C-terminal region of human ACAT1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACAT1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACAT1
Synonyms ACAT, MAT
Function This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed:1715688, PubMed:7728148, PubMed:9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed:1715688, PubMed:7728148, PubMed:9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed:17371050). Thereby, it plays a major role in ketone body metabolism (PubMed:17371050, PubMed:1715688, PubMed:7728148, PubMed:9744475).
Cellular Location Mitochondrion.
Research Areas
Citations (0)
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Background

ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

References

Locke,J.A.,Prostate 68 (1), 20-33 (2008)
Guo,Z.Y.,Biochemistry 46 (35), 10063-10071 (2007)
Haapalainen,A.M.,Biochemistry 46 (14), 4305-4321 (2007)

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$ 370.00
Cat# AW5517-U100
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