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USO1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • IHC-P - USO1 Antibody (C-term) AW5531-U200
    USO1 Antibody (C-term) (AW5531)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • WB - USO1 Antibody (C-term) AW5531-U200
    All lanes : Anti-USO1 Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60763
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=108,109 KDa
Additional Information
Gene ID 8615
Antigen Region 932-960 aa
Other Names General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP
Dilution IHC-P~~1:10~50
Target/Specificity This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSO1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USO1
Synonyms VDP
Function General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity).
Cellular Location Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes
Research Areas
Citations (0)

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The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.


Striegl, H., et al. PLoS ONE 5 (2), E8991 (2010) :
Merk, M., et al. J. Immunol. 182(11):6896-6906(2009)
Mukherjee, S., et al. J. Biol. Chem. 284(3):1709-1717(2009)
Striegl, H., et al. PLoS ONE 4 (2), E4656 (2009) :
Guo, Y., et al. Mol. Biol. Cell 19(7):2830-2843(2008)

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$ 325.00
$ 110.00
Cat# AW5531-U200
(40 western blots)
Availability: In Stock
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