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LDHA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - LDHA Antibody (Center) AW5539-U200
    All lanes : Anti-LDHA Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: A375 whole cell lysate Lane 3: human placenta lysate Lane 4: COS-7 whole cell lysate Lane 5: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - LDHA Antibody (Center) AW5539-U200
    Formalin-fixed and paraffin-embedded human skin tissue reacted with LDHA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - LDHA Antibody (Center) AW5539-U200
    LDHA Antibody (Center) (Cat. #AW5539) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00338
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=40,30,27,37;R=36 KDa
Additional Information
Gene ID 3939
Antigen Region 154-182 aa
Other Names L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
Dilution WB~~1:1000
Target/Specificity This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the Central region of human LDHA.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLDHA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria.


Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009)
Xie, H., et al. Mol. Cancer Ther. 8(3):626-635(2009)
Koukourakis, M.I., et al. Oncology 77(5):285-292(2009)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)

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$ 325.00
$ 110.00
Cat# AW5539-U200
(40 western blots)
Availability: In Stock
Bulk Size
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