5-Hydroxytryptamine Receptor 1B (extracellular) Antibody
Affinity purified rabbit polyclonal antibody
|Calculated MW||R=43 KDa|
|Antigen Region||33-49 aa|
|Other Names||5-hydroxytryptamine receptor 1B, 5-HT-1B, 5-HT1B, S12, Serotonin 1D beta receptor, 5-HT-1D-beta, Serotonin receptor 1B, HTR1B, HTR1DB|
|Target/Specificity||Peptide CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine Receptor 1B (Accession P28222). Extracellular, N-terminus.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||5-Hydroxytryptamine Receptor 1B (extracellular) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Detected in cerebral artery smooth muscle cells (at protein level). Detected in brain cortex, striatum, amygdala, medulla, hippocampus, caudate nucleus and putamen|
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Provided below are standard protocols that you may find useful for product applications.
Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rthythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2. To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-Protein Coupled Receptor superfamily and therefore span the membrane seven times3. The 5-Hydroxytryptamine Receptor 1B (5-HT1B) is involved in several physiological behaviors and psychiatric disease, including locomotor activity, drug abuse reinforcement, migraine, anxiety states and aggressive behaviors1. Pharmacological studies suggest that 5-HT1B is expressed by both serotonergic and non-serotonergic neurons. In the nervous system, this receptor is located in the axon terminal of the plasma membrane of unmyelinated axons. It is predominantly localized on pre-synaptic membranes. Signal transduction studies of the receptor indicate that this receptor is coupled negatively to adenylyl cyclase as its activation induces a decrease in adenylyl cyclase activity1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human 5-Hydroxytryptamine Receptor 1B (5-HT1B). Anti-5-Hydroxytryptamine Receptor 1B (extracellular) antibody (#AG1011) can be used for western blot analysis. It has been designed to recognize 5-Hydroxytryptamine Receptor 1B from human, rat and mouse samples.
1. Sari, Y. (2004) Neurosi. Biobehav. Rev. 28, 565.
2. Berger, M. et al. (2009) Annu. Rev. Med. 60, 355.
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