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ACTG1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ACTG1 Antibody (Center) AW5546-U200
    All lanes : Anti-ACTG1 Antibody (Center) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • FC - ACTG1 Antibody (Center) AW5546-U200
    Flow cytometric analysis of K562 cells using ACTG1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - ACTG1 Antibody (Center) AW5546-U200
    Confocal immunofluorescent analysis of ACTG1 Antibody (Center)(Cat#AW5546) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, WB
Primary Accession P63261
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=42 KDa
Additional Information
Gene ID 71
Antigen Region 188-215 aa
Other Names Actin, cytoplasmic 2, Gamma-actin, Actin, cytoplasmic 2, N-terminally processed, ACTG1, ACTG
Dilution WB~~1:1000
FC~~1:10~50
IF~~1:10~50
Target/Specificity This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of human ACTG1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTG1
Synonyms ACTG
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)

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Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.

References

de Heer,A.M., Ann. Otol. Rhinol. Laryngol. 118 (5), 382-390 (2009)
Mouilleron,S., EMBO J. 27 (23), 3198-3208 (2008)
Liu,P., J Genet Genomics 35 (9), 553-558 (2008)

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$ 325.00
$ 110.00
Cat# AW5546-U200
Size:
Quantity:
(40 western blots)
Availability: In Stock
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