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SNRPA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - SNRPA Antibody (Center) AW5557-U200
    All lanes : Anti-SNRPA Antibody (Center) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: CEM whole cell lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - SNRPA Antibody (Center) AW5557-U200
    SNRPA Antibody (Center) (AW5557)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SNRPA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09012
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=31;M=32 KDa
Additional Information
Gene ID 6626
Antigen Region 83-111 aa
Other Names U1 small nuclear ribonucleoprotein A, U1 snRNP A, U1-A, U1A, SNRPA
Dilution WB~~1:1000
Target/Specificity This SNRPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human SNRPA.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNRPA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
Cellular Location Nucleus.
Citations (0)

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The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.


Guan, F., et al. RNA 13(12):2129-2140(2007)
Hall-Pogar, T., et al. RNA 13(7):1103-1115(2007)
Shajani, Z., et al. J. Mol. Biol. 349(4):699-715(2005)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Jessen, T.H., et al. EMBO J. 10(11):3447-3456(1991)

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$ 325.00
$ 110.00
Cat# AW5557-U200
(40 western blots)
Availability: In Stock
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