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BMI1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - BMI1 Antibody (C-term) AW5563-U200
    All lanes : Anti-BMI1 Antibody (C-term) at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: MCF-7 whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - BMI1 Antibody (C-term) AW5563-U200
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 with AW5563 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB
Primary Accession P35226
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=37;M=37 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 100532731;648
Antigen Region 281-314 aa
Other Names Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51
Dilution WB~~1:1000
IF~~1:25
Target/Specificity This BMI1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-314 amino acids from the C-terminal region of human BMI1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBMI1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BMI1
Synonyms PCGF4, RNF51
Function Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Cellular Location Nucleus. Cytoplasm.
Research Areas
Citations (0)

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Background

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

References

Alkema M.J.,et al.Hum. Mol. Genet. 2:1597-1603(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Levy L.S.,et al.Oncogene 8:1833-1838(1993).

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$ 325.00
$ 110.00
Cat# AW5563-U200
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Quantity:
(40 western blots)
Availability: In Stock
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