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FN3KRP Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC - FN3KRP Antibody (N-Term) AW5569-U200
    AW5569 staining FN3KRP in human Stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - FN3KRP Antibody (N-Term) AW5569-U200
    All lanes : Anti-FN3KRP Antibody (N-Term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9HA64
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=34;M=34 KDa
Additional Information
Gene ID 79672
Antigen Region 24-58 aa
Other Names Ketosamine-3-kinase, 271-, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, FN3KRP
Dilution IHC~~1:25
WB~~1:2000
Target/Specificity This FN3KRP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 24-58 amino acids from human FN3KRP.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFN3KRP Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FN3KRP
Function Phosphorylates psicosamines and ribulosamines, but not fructosamines, on the third carbon of the sugar moiety. Protein- bound psicosamine 3-phosphates and ribulosamine 3-phosphates are unstable and decompose under physiological conditions. Thus phosphorylation leads to deglycation.
Tissue Location Expressed in erythrocytes.
Research Areas
Citations (0)

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Background

Phosphorylates psicosamines and ribulosamines, but not fructosamines, on the third carbon of the sugar moiety. Protein- bound psicosamine 3-phosphates and ribulosamine 3-phosphates are unstable and decompose under physiological conditions. Thus phosphorylation leads to deglycation.

References

Collard F.,et al.Diabetes 52:2888-2895(2003).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Collard F.,et al.Biochem. J. 382:137-143(2004).
Oppermann F.S.,et al.Mol. Cell. Proteomics 8:1751-1764(2009).

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$ 325.00
$ 110.00
Cat# AW5569-U200
Size:
Quantity:
(40 western blots)
Availability: In Stock
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