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TAF8 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TAF8 Antibody (Center) AW5590-U100
    All lanes : Anti-TAF8 Antibody (Center) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - TAF8 Antibody (Center) AW5590-U100
    TAF8 Antibody (Center) (Cat. #AW5590)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TAF8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB
Primary Accession Q7Z7C8
Other Accession A7MAZ4, Q5ZMS1
Reactivity Human
Predicted Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=34,37;M=29,34,35,24 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 129685
Antigen Region 151-178 aa
Other Names Transcription initiation factor TFIID subunit 8, Protein taube nuss, TBP-associated factor 43 kDa, TBP-associated factor 8, Transcription initiation factor TFIID 43 kDa subunit, TAFII-43, TAFII43, hTAFII43, TAF8, TAFII43, TBN
Dilution WB~~1:2000
IHC-P~~1:10~50
Target/Specificity This TAF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-178 amino acids from the Central region of human TAF8.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF8
Synonyms TAFII43, TBN
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473). TAF8 is involved in forming the TFIID-B module, together with TAF5 (PubMed:33795473). Mediates both basal and activator-dependent transcription (PubMed:14580349). Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts (PubMed:14580349). Required for the integration of TAF10 in the TAF complex (PubMed:14580349). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity).
Cellular Location Nucleus. Cytoplasm Note=Predominantly nuclear.
Citations (0)
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Background

This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].

References

Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009)
Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Soutoglou, E., et al. Mol. Cell. Biol. 25(10):4092-4104(2005)
Guermah, M., et al. Mol. Cell 12(4):991-1001(2003)

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$ 370.00
Cat# AW5590-U100
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