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PSMB7 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC - PSMB7 Antibody (Center) AW5615-U200
    AW5615 staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - PSMB7 Antibody (Center) AW5615-U200
    All lanes : Anti-PSMB7 Antibody (Center) at 1:8000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q99436
Other Accession Q2TBP0, P70195, Q9JHW0
Reactivity Human, Mouse
Predicted Human, Dog, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=30;M=30;R=30 KDa
Additional Information
Gene ID 5695
Antigen Region 180-211 aa
Other Names Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, PSMB7, Z
Dilution IHC~~1:25
WB~~1:8000
Target/Specificity This PSMB7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 180-211 amino acids from the Central region of human PSMB7.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB7
Synonyms Z
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809, ECO:0000269|PubMed:18549262}. Nucleus
Tissue Location Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues
Research Areas
Citations (0)

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Background

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.

References

Hisamatsu H.,et al.J. Exp. Med. 183:1807-1816(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.

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$ 325.00
$ 110.00
Cat# AW5615-U200
Size:
Quantity:
(40 western blots)
Availability: In Stock
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