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HTR1E Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC - HTR1E Antibody (C-Term) AW5621-U200
    AW5621 staining HTR1E in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - HTR1E Antibody (C-Term) AW5621-U200
    All lanes : Anti-HTR1E Antibody (C-Term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: human brain lysate Lane 3: Jurkat whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P28566
Other Accession Q6VB83, Q9N2B6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=42 KDa
Additional Information
Gene ID 3354
Antigen Region 223-258 aa
Other Names 5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E
Dilution IHC~~1:25
WB~~0.25
Target/Specificity This HTR1E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 223-258 amino acids from human HTR1E.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHTR1E Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HTR1E
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
Cellular Location Cell membrane; Multi- pass membrane protein
Tissue Location Detected in brain.
Research Areas
Citations (0)

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Background

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.

References

McAllister G.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:5517-5521(1992).
Levy F.O.,et al.FEBS Lett. 296:201-206(1992).
Zgombick J.M.,et al.Mol. Pharmacol. 42:180-185(1992).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).

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$ 325.00
$ 110.00
Cat# AW5621-U200
Size:
Quantity:
(40 western blots)
Availability: In Stock
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