|Calculated MW||H=118,66;M=118 KDa|
|Antigen Region||861-889 aa|
|Other Names||Ubiquitin-like modifier-activating enzyme 6, Ubiquitin-activating enzyme 6, Monocyte protein 4, MOP-4, Ubiquitin-activating enzyme E1-like protein 2, E1-L2, UBA6, MOP4, UBE1L2|
|Target/Specificity||This UBA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 861-889 amino acids from the C-terminal region of human UBA6.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBA6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin- E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.|
|Tissue Location||Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).
Groettrup, M., et al. Trends Biochem. Sci. 33(5):230-237(2008)
Chiu, Y.H., et al. Mol. Cell 27(6):1014-1023(2007)
Pelzer, C., et al. J. Biol. Chem. 282(32):23010-23014(2007)
Jin, J., et al. Nature 447(7148):1135-1138(2007)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
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