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UBA6 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - UBA6 Antibody (C-term) AW5631-U200
    All lanes : Anti-UBA6 Antibody (C-term) at 1:2000 dilution Lane 1: Daudi whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A0AVT1
Other Accession Q8C7R4
Reactivity Human, Mouse
Predicted Dog
Host Rabbit
Clonality Polyclonal
Calculated MW H=118,66;M=118 KDa
Additional Information
Gene ID 55236
Antigen Region 861-889 aa
Other Names Ubiquitin-like modifier-activating enzyme 6, Ubiquitin-activating enzyme 6, Monocyte protein 4, MOP-4, Ubiquitin-activating enzyme E1-like protein 2, E1-L2, UBA6, MOP4, UBE1L2
Dilution WB~~0.25
Target/Specificity This UBA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 861-889 amino acids from the C-terminal region of human UBA6.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBA6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBA6
Synonyms MOP4, UBE1L2
Function Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin- E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
Tissue Location Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
Research Areas
Citations (0)

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Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).


Groettrup, M., et al. Trends Biochem. Sci. 33(5):230-237(2008)
Chiu, Y.H., et al. Mol. Cell 27(6):1014-1023(2007)
Pelzer, C., et al. J. Biol. Chem. 282(32):23010-23014(2007)
Jin, J., et al. Nature 447(7148):1135-1138(2007)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)

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$ 325.00
$ 110.00
Cat# AW5631-U200
(40 western blots)
Availability: In Stock
Bulk Size
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