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HNRPAB Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - HNRPAB Antibody (N-term) AW5652-U200
    All lanes : Anti-HNRPAB Antibody (N-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - HNRPAB Antibody (N-term) AW5652-U200
    Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term)(Cat#AW5652).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.
  • IHC-P - HNRPAB Antibody (N-term) AW5652-U200
    HNRPAB Antibody (N-term) (Cat. #AW5652) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPAB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99729
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 36 KDa
Additional Information
Gene ID 3182
Antigen Region 1-30 aa
Other Names Heterogeneous nuclear ribonucleoprotein A/B, hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, HNRNPAB, ABBP1, HNRPAB
Dilution WB~~1:1000
Target/Specificity This HNRPAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HNRPAB.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHNRPAB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ABBP1, HNRPAB
Function Binds single-stranded RNA. Has a high affinity for G- rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.
Cellular Location Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Tissue Location Ubiquitous.
Citations (0)

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This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are produced by RNA polymerase II and are components of the heterogeneous nuclear RNA (hnRNA) complexes. They are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene, which binds to one of the components of the multiprotein editosome complex, has two repeats of quasi-RRM (RNA recognition motif) domains that bind to RNAs.


Jonson, L., et al. Mol. Cell Proteomics 6(5):798-811(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Ong, S.E., et al. Nat. Methods 1(2):119-126(2004)

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$ 315.00
$ 110.00
Cat# AW5652-U200
(40 western blots)
Availability: In Stock
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