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FLAG tag antibody (DYKDDDDK)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - FLAG tag antibody (DYKDDDDK) AW5654-U200
    All lanes : Anti-FLAG tag antibody at 1:1000 dilution Lane 1: 293T/17 transfected with 12tag lysate (10ug) Lane 2: Non-transfected 293T/17 lysate (10ug) Lane 3: 12tag recombinant protein lysate (0. 01ug) Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rabbit
Clonality Polyclonal
Calculated MW 45-50KD
Additional Information
Other Names Tag from influenza hemagglutinin protein
Dilution WB~~1:1000
Target/Specificity KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFLAG tag antibody (DYKDDDDK) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).


Chubet RG, Brizzard BL. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 1996;20(1):136-141

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$ 315.00
$ 110.00
Cat# AW5654-U200
(40 western blots)
Availability: In Stock
Bulk Size
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