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HA Tag Antibody

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - HA Tag Antibody AW5659-U200
    All lanes : Anti-HA Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower) Lane 1: 293T/17 transfected with 12tag lysate (1ug) Lane 2: Non-transfected 293T/17 lysate (1ug) Lane 3: 12tag recombinant protein lysate (0. 1ug) Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rabbit
Clonality Polyclonal
Calculated MW 45-50 KDa
Additional Information
Antigen Region N/A aa
Other Names Glutathione S-transferase
Dilution WB~~1:1000
Target/Specificity KLH conjugated synthetic peptide encoding HA tag was used as antigen.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.


Marco., J Biol Chem. (2009) February 6; 284(6): 3577 585. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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$ 315.00
$ 110.00
Cat# AW5659-U200
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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