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FLAG tag antibody (DYKDDDDK)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - FLAG tag antibody (DYKDDDDK) AW5662-U100
    All lanes : Anti-FLAG tag antibody at 1:1000 dilution Lane 1: 293T/17 transfected with 12tag lysate (10ug) Lane 2: Non-transfected 293T/17 lysate (10ug) Lane 3: 12tag recombinant protein lysate (1ug) Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Host Rabbit
Clonality Polyclonal
Calculated MW 45-50KD
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Antigen Region N/A aa
Other Names Tag from influenza hemagglutinin protein
Dilution WB~~1:1000
Target/Specificity KLH conjugated synthetic peptide was used as antigen.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFLAG tag antibody (DYKDDDDK) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).

References

Chubet RG, Brizzard BL. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 1996;20(1):136-141

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$ 370.00
Cat# AW5662-U100
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