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RPL14 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • FC - RPL14 Antibody (C-Term) AW5674-U200
    Overlay histogram showing U-2OS cells stained with AW5674 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5674, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RPL14 Antibody (C-Term) AW5674-U200
    All lanes : Anti-RPL14 Antibody (C-Term) at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: U-2OS whole cell lysate Lane 7: mouse spleen lysate Lane 8: rat spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50914
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=23;M=24;R=23 KDa
Additional Information
Gene ID 9045
Antigen Region 173-204 aa
Other Names 60S ribosomal protein L14, CAG-ISL 7, RPL14
Dilution FC~~1:25
Target/Specificity This RPL14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 173-204 amino acids from human RPL14.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL14 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL14
Citations (0)

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Aoki M.,et al.Diabetes 45:157-164(1996).
Tanaka M.,et al.Biochem. Biophys. Res. Commun. 243:531-537(1998).
Yoshihama M.,et al.Genome Res. 12:379-390(2002).
Lin L.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

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$ 315.00
$ 110.00
Cat# AW5674-U200
(40 western blots)
Availability: In Stock
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