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RPS7 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - RPS7 Antibody (N-Term) AW5678-U200
    Overlay histogram showing Hela cells stained with AW5678 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5678, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - RPS7 Antibody (N-Term) AW5678-U200
    All lanes : Anti-RPS7 Antibody (N-Term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: human spleen lysate Lane 7: mouse spleen lysate Lane 8: human liver lysate Lane 9: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P62081
Other Accession A6H769, P62084, Q5RT64, P62082, P62083, P50894, P02362
Reactivity Human, Mouse
Predicted Human, Dog, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW H=22;M=22 KDa
Additional Information
Gene ID 6201
Antigen Region 7-40 aa
Other Names 40S ribosomal protein S7, RPS7
Dilution FC~~1:25
WB~~1:1000
Target/Specificity This RPS7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 7-40 amino acids from human RPS7.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS7 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS7
Function Required for rRNA maturation.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with NEK6 in the centrosome
Citations (0)

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Background

Required for rRNA maturation.

References

Annilo T.,et al.Gene 165:297-302(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Vladimirov S.N.,et al.Eur. J. Biochem. 239:144-149(1996).

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$ 315.00
$ 110.00
Cat# AW5678-U200
Size:
Quantity:
(40 western blots)
Availability: In Stock
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