Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Immune system process   >   PD L1 Monoclonal Antibody (PDL1)   

PD L1 Monoclonal Antibody (PDL1)

Purified Mouse Monoclonal Antibody (Mab)

     
crown
Products labeled with a "crown" meet aggressive quality standards. Learn more.
  • WB - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    All lanes : Anti-PDL-1 Antibody at 0.5-1µg/ml dilution Lane 1: 293T/PDL1 whole cell lysate Lane 2: 293T whole cell lysate Lane 3: NCI-H1975 whole cell lysate Lane 4: MDA-MB231 whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: NCI-H226 whole cell lysate Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L),Peroxidase conjµgated at 1/5000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by abgent test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Dako test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Leica test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of PDL-1 in human tonsil tissue sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by abgent test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of PDL-1 in human tonsil tissue sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Dako test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of PDL-1 in MCF-7 cell (left) and NCI-H226(right) cell sections by abgent test . Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of PDL-1 in untransfected(left) or transfected(right) with 293T cell sections by abgent test . Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    AW5698 staining PD-L1 in human lung squamous carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Overlay histogram showing isotype control(blue line), 293T-PDL1(green line), MDA-MB-231(red line), NCI-H226(black line) cells stained with PD L1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NH174309) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
    detail
  • IHC - PD L1 Monoclonal  Antibody (PDL1) AW5698-U100
    Immunohistochemical analysis of paraffin-embedded Human tonsil section using PDL1(Cat#AW5698). AW5698 was diluted at 1:500 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, IHC-P-Leica
Primary Accession Q9NZQ7
Reactivity Human
Predicted Human
Host Mouse
Clonality monoclonal
Calculated MW 33275 Da
Isotype IgG1
Antigen Source HUMAN
Additional Information
Gene ID 29126
Other Names Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, PDL-1
Dilution WB~~1:500-1:1000
IHC-P~~1:100
IHC-P-Leica~~1:100-1:600
FC~~1:25
IHC~~1:500
Target/Specificity This PD L1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 256-290 amino acids from the human region of human PD L1.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPD L1 Monoclonal Antibody (PDL1) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD274 (HGNC:17635)
Function Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed:32929201).
Cellular Location Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Nucleus. Note=Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation (PubMed:28813417). Translocates to the nucleus in response to hypoxia via its interaction with phosphorylated STAT3 (PubMed:32929201). [Isoform 2]: Endomembrane system; Single-pass type I membrane protein
Tissue Location Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Research Areas
Citations ( 0 )

Background

Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

References

Dong H.,et al.Nat. Med. 5:1365-1369(1999).
Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000).
He X.-H.,et al.Acta Pharmacol. Sin. 26:462-468(2005).
Chi X.-Y.,et al.Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 380.00
Cat# AW5698-U100
Size:
Quantity:
Availability: In Stock
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"