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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   SYP Antibody(C-term)   

SYP Antibody(C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SYP Antibody(C-term) AX10004
    SYP Antibody (C-term) (Cat. #AX10004) western blot analysis in mouse brain and cerebellum tissue lysates (35ug/lane).This demonstrates the SYP antibody detected the SYP protein (arrow).
  • IHC-P - SYP Antibody(C-term) AX10004
    SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IHC-P - SYP Antibody(C-term) AX10004
    SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse hippocampus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - SYP Antibody(C-term) AX10004
    Confocal immunofluorescent analysis of SYP Antibody (C-term) with human brain tissue followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • IF - SYP Antibody(C-term) AX10004
    Confocal immunofluorescent analysis of SYP Antibody (C-term) with mouse brain tissue followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - SYP Antibody(C-term) AX10004
    SYP Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P08247
Other Accession P07825, Q62277, NP_003170.1
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Clone Names RB32966
Calculated MW 33845 Da
Antigen Region 225-253 aa
Additional Information
Gene ID 6855
Other Names Synaptophysin, Major synaptic vesicle protein p38, SYP
Target/Specificity This SYP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from the C-terminal region of human SYP.
Dilution WB~~1:2000
IHC-P~~1:1000
IF~~1:100
FC~~1:50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSYP Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SYP
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome
Tissue Location Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype
Research Areas
Citations (0)

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Background

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM].

References

Ishida, M., et al. Oncol. Rep. 22(4):733-737(2009) Sterlacci, W., et al. Virchows Arch. 455(2):125-132(2009) Head, E., et al. Neurobiol. Aging 30(7):1125-1134(2009) Gulubova, M., et al. J. Gastroenterol. Hepatol. 23(10):1574-1585(2008) Glantz, L.A., et al. Neuroscience 149(3):582-591(2007)

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$ 295.00
$ 99.00
Cat# AX10004
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(40 western blots)
Availability: In Stock
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