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(DANRE) zte25 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (DANRE) zte25 Antibody (N-term) Azb18715a
    Western blot analysis of lysates from ZF4 cell line, zebra fish muscle, Zebrafish tissue lysate(from left to right), using (DANRE) zte25 Antibody (N-term)(Cat. #Azb18715a). Azb18715a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession A6H8T7
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Clone/Animal Names RB47039
Calculated MW 82236 Da
Antigen Region 92-126 aa
Additional Information
Other Names Cytosolic carboxypeptidase 2, 3417-, ATP/GTP-binding protein-like 2, Testis-expressed protein 25, zte25, agbl2, ccp2
Target/Specificity This (DANRE) zte25 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-126 amino acids of DANRE zte25.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(DANRE) zte25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name zte25
Synonyms agbl2, ccp2
Function Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin.
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8CDK2}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q5U5Z8}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q5U5Z8}. Note=Colocalizes with gamma- tubulin in the centrioles and with glutamylated tubulin in the basal bodies of ciliated cells. {ECO:0000250|UniProtKB:Q5U5Z8}
Research Areas
Citations (0)
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Background

Metallocarboxypeptidase involved in the detyrosination of alpha-tubulin C-terminus (By similarity).

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$ 365.00
$ 140.00
Cat# Azb18715a
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