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(DANRE) mapk12 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - (DANRE) mapk12 Antibody (C-term) Azb18716c
    Western blot analysis of lysates from zebra fish brain, Zebrafish tissue lysate (from left to right), using (DANRE) mapk12 Antibody (C-term)(Cat. #Azb18716c). Azb18716c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O42376
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB47232
Calculated MW 41971 Da
Additional Information
Other Names Mitogen-activated protein kinase 12, MAP kinase 12, MAPK 12, Stress-activated protein kinase 3, mapk12, sapk3
Target/Specificity This (DANRE) mapk12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 319-353 amino acids from the C-terminal region of human (DANRE) mapk12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(DANRE) mapk12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name mapk12
Synonyms sapk3
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets (By similarity).
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets (By similarity).

References

Goedert M.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# Azb18716c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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