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(DANRE) tie2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (DANRE) tie2 Antibody (N-term) Azb21568a
    Anti-ROBO1 Antibody (Y932)at 1:2000 dilution + ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 122 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O73791
Reactivity Zebrafish
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Clone Names RB30729
Calculated MW 122361 Da
Additional Information
Gene ID 30747
Other Names Tyrosine-protein kinase receptor Tie-2, Tyrosine kinase with Ig and EGF homology domains-2, tie2, tie-2
Target/Specificity This (DANRE) tie2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 160-194 amino acids from the N-terminal region of DANRE tie2.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(DANRE) tie2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name tie2
Synonyms tie-2
Function Tyrosine-protein kinase that acts as cell-surface receptor for angiopoietins and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Can activate or inhibit angiogenesis, depending on the context. Angiopoietin signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cell junction. Cell junction, focal adhesion Cytoplasm, cytoskeleton. Note=Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell (By similarity)
Tissue Location Expressed in most populations of endothelial cells in 24 hours embryos. Not present in intersegmental vessels
Research Areas
Citations (0)

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Background

Tyrosine-protein kinase that acts as cell-surface receptor for angiopoietins and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Can activate or inhibit angiogenesis, depending on the context. Angiopoietin signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors (By similarity).

References

Lyons M.S.,et al.Dev. Dyn. 212:133-140(1998).

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$ 99.00
Cat# Azb21568a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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