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(DANRE) tie2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O73791 |
| Reactivity | Zebrafish |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Clone/Animal Names | RB30729 |
| Calculated MW | 122361 Da |
| Antigen Region | 160-194 aa |
| Gene ID | 30747 |
|---|---|
| Other Names | Tyrosine-protein kinase receptor Tie-2, Tyrosine kinase with Ig and EGF homology domains-2, tie2, tie-2 |
| Target/Specificity | This (DANRE) tie2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 160-194 amino acids from the N-terminal region of DANRE tie2. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | (DANRE) tie2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | tek {ECO:0000250|UniProtKB:Q02763} |
|---|---|
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for angiopoietins and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Can activate or inhibit angiogenesis, depending on the context. Angiopoietin signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:Q02763}; Single-pass type I membrane protein. Cell junction {ECO:0000250|UniProtKB:Q02763}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q02763}. Cytoplasm, cytoskeleton Note=Recruited to cell-cell contacts in quiescent endothelial cells (By similarity). Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell (By similarity) {ECO:0000250|UniProtKB:Q02763} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Tyrosine-protein kinase that acts as cell-surface receptor for angiopoietins and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Can activate or inhibit angiogenesis, depending on the context. Angiopoietin signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors (By similarity).
References
Lyons M.S.,et al.Dev. Dyn. 212:133-140(1998).
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