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PEMT Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9UBM1 |
|---|---|
| Clone Names | 70411220 |
| Gene ID | 10400 |
|---|---|
| Other Names | Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP1025b was selected from the C-term region of human PEMT. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | PEMT {ECO:0000255|HAMAP-Rule:MF_03216} |
|---|---|
| Synonyms | PEMPT, PNMT |
| Function | Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PubMed:12431977, PubMed:15927961). Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3- phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N- dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2- diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L- homocysteine in each step (PubMed:12431977, PubMed:15927961). Responsible for approximately 30% of hepatic PC with the CDP-choline pathway accounting for the other 70% (Probable). |
| Cellular Location | Endoplasmic reticulum. Note=localized in the endoplasmic reticulum (ER) of the liver and in a lipid metabolism-rich region of the ER known as mitochondria-associated membranes (PubMed:15927961) Adopts a topography within the ER membrane that positions both termini in the cytosol (PubMed:12431977). [Isoform 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03216} |
| Tissue Location | Primarily expressed in liver (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
PEMT is an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17.
References
Walkey C.J., Biochim. Biophys. Acta 1436:405-412(1999).Shields D.J., Biochim. Biophys. Acta 1532:105-114(2001).Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
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