|Other Accession||NP_001182145.1, NP_659414.2, NP_001182144.1|
|Other Names||DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide exchange factor (GEF) for RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. May play a role in clathrin- mediated endocytosis.|
|Cellular Location||Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle|
|Tissue Location||Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T- cells as compared with naive T-cells|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Marat, A.L., et al. J. Biol. Chem. 285(14):10627-10637(2010)Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44(2010)Venter, J.C., et al. Science 291(5507):1304-1351(2001)
Clathrin (see MIM 118955)-mediated endocytosis is a majormechanism for internalization of proteins and lipids. Members ofthe connecdenn family, such as DENND1B, function as guaninenucleotide exchange factors (GEFs) for the early endosomal smallGTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptorprotein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35activation with the clathrin machinery (Marat and McPherson, 2010[PubMed 20154091]).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.