|Other Accession||NP_001182145.1, NP_659414.2, NP_001182144.1|
|Other Names||DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells (PubMed:20154091, PubMed:20937701, PubMed:24520163, PubMed:26774822). Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form (PubMed:20154091, PubMed:20937701). Plays a role in clathrin- mediated endocytosis (PubMed:20154091). Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity (PubMed:26774822). Dysregulation leads to impaired TCR down- modulation and recycling, affecting cytokine production in TH2 cells (PubMed:26774822).|
|Cellular Location||Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin- coated vesicle|
|Tissue Location||Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T- cells as compared with naive T-cells|
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Provided below are standard protocols that you may find useful for product applications.
Marat, A.L., et al. J. Biol. Chem. 285(14):10627-10637(2010)Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44(2010)Venter, J.C., et al. Science 291(5507):1304-1351(2001)
Clathrin (see MIM 118955)-mediated endocytosis is a majormechanism for internalization of proteins and lipids. Members ofthe connecdenn family, such as DENND1B, function as guaninenucleotide exchange factors (GEFs) for the early endosomal smallGTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptorprotein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35activation with the clathrin machinery (Marat and McPherson, 2010[PubMed 20154091]).
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