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MBD3 Antibody (C-term C215) Blocking Peptide

Synthetic peptide

Product Information
Primary Accession O95983
Clone Names 2103112
Peptide ID 2103112
Additional Information
Gene ID 53615
Other Names Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3
Target/Specificity The synthetic peptide sequence used to generate the antibody AP1038b was selected from the C-term region of human MBD3 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Format The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Protein Information
Name MBD3
Function Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.
Cellular Location Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes
Research Areas
Citations (0)

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DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently foundto be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol.MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA andbelieved to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stemcells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.


Bhattacharya SK, Ramchandani S, Cervoni N, Szyf. M. Nature, 397 (6720):579-583 1999.Hendrich B and Bird A. Mol Cell Biol, 18: 6538-6547(1998).Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A. J Biol Chem 275 (42): 32422-32429 (2000).Bader S, Walker M, Harrison D. Br J Cancer 83(12): 1646-1649 (2000).

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$ 80.00
Cat# BP1038b
(40 western blots)
Availability: In Stock
Bulk Size
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