|Other Names||DNA (cytosine-5)-methyltransferase 3-like, Dnmt3l|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1062a was selected from the region of human Mouse Dnmt3L. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases.|
|Tissue Location||Expressed in testis, thymus, ovary, and heart.|
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mDnmt3L (DNA methyltransferase 3L) does not appear to be catalytically active due to loss of the critical active site residues. It may function not directly as a DNA methyltransferase but rather as a regulator of methylation at imprinted loci. It is required specifically for the establishment of genomic imprints but is dispensable for their propagation. It is essential for the de novo methylation of single-copy DNA sequences. The protein is well-express in testis, thymus, ovary, and heart. mDnmt3L, which belongs to the 5-cytosine methyltransferase family, contains 1 ADD-type zinc finger.
Okazaki, Y., et al., Nature 420(6915):563-573 (2002).Bourc'his, D., et al., Science 294(5551):2536-2539 (2001).Aapola, U., et al., Cytogenet. Cell Genet. 92 (1-2), 122-126 (2001).
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