ACF Antibody (C-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9NQ94 |
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Clone Names | 81201001 |
Gene ID | 29974 |
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Other Names | APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312|EMBL:CAB947541} |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | A1CF |
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Synonyms | ACF, ASP {ECO:0000312|EMBL:CAB94754.1} |
Function | Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense- mediated decay. |
Cellular Location | Nucleus. Endoplasmic reticulum Cytoplasm. Note=Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1. |
Tissue Location | Widely expressed with highest levels in brain, liver, pancreas, colon and spleen. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mammalian apolipoprotein B mRNA undergoes site-specific Cto U deamination, which is mediated by a multi-component enzymecomplex containing a minimal core composed of APOBEC-1 and acomplementation factor encoded by this gene. The gene product hasthree non-identical RNA recognition motifs and belongs to the hnRNPR family of RNA-binding proteins. It has been proposed that thiscomplementation factor functions as an RNA-binding subunit anddocks APOBEC-1 to deaminate the upstream cytidine. Studies suggestthat the protein may also be involved in other RNA editing or RNAprocessing events.
References
Galloway, C.A., et al. Biochem. Biophys. Res. Commun. 391(1):659-663(2010)Blanc, V., et al. Mol. Cell. Biol. 25(16):7260-7269(2005)Deloukas, P., et al. Nature 429(6990):375-381(2004)Xie, K., et al. Proc. Natl. Acad. Sci. U.S.A. 101(21):8114-8119(2004)Blanc, V., et al. J. Biol. Chem. 278(42):41198-41204(2003)
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