|Other Names||Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1067a was selected from the N-term region of human MBD2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.|
|Cellular Location||Nucleus. Note=Nuclear, in discrete foci Detected at replication foci in late S phase|
|Tissue Location||Highly expressed in brain, heart, kidney, stomach, testis and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Zhu, Y., et al., Cancer 100(9):1853-1858 (2004).Ghoshal, K., et al., J. Biol. Chem. 279(8):6783-6793 (2004).Fujita, H., et al., Mol. Cell. Biol. 23(8):2645-2657 (2003).Patra, S.K., et al., Biochem. Biophys. Res. Commun. 302(4):759-766 (2003).Lembo, F., et al., Mol. Cell. Biol. 23(5):1656-1665 (2003).
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