|Other Names||tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5- methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.|
|Cellular Location||Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Note=Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA|
|Tissue Location||Expressed in adult and fetal brain and in lymphoblastoid cells.|
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Provided below are standard protocols that you may find useful for product applications.
Maturation of cytoplasmic tRNAs includes splicing ofintrons, which are located 1 nucleotide 3-prime from the anticodonin all intron-containing tRNA genes. In tRNA-leu(CAA), the firstposition of the anticodon, C34, is converted to 5-methylcytosine, amodification necessary to stabilize the anticodon-codon pairing andcorrectly translate the mRNA. NSUN2 encodes a methyltransferasethat catalyzes the intron-dependent formation of 5-methylcytosineat C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed17071714]).
Bailey, S.D., et al. Diabetes Care (2010) In press :Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Frye, M., et al. Cancer Lett. 289(1):71-80(2010)Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)Hussain, S., et al. J. Cell Biol. 186(1):27-40(2009)
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