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CAD Antibody (Center) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P27708 |
|---|---|
| Clone Names | 80411160 |
| Gene ID | 790 |
|---|---|
| Other Names | CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase, CAD |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | CAD (HGNC:1424) |
|---|---|
| Function | Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5), aspartate transcarbamylase (ATCase; EC 2.1.3.2) and dihydroorotase (DHOase; EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate. |
| Cellular Location | Cytoplasm. Nucleus. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The de novo synthesis of pyrimidine nucleotides isrequired for mammalian cells to proliferate. This gene encodes atrifunctional protein which is associated with the enzymaticactivities of the first 3 enzymes in the 6-step pathway ofpyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II),aspartate transcarbamoylase, and dihydroorotase. This protein isregulated by the mitogen-activated protein kinase (MAPK) cascade,which indicates a direct link between activation of the MAPKcascade and de novo biosynthesis of pyrimidine nucleotides.
References
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Ahuja, V., et al. J. Inherit. Metab. Dis. 31(4):481-491(2008)Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)Olsen, J.V., et al. Cell 127(3):635-648(2006)
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