|Other Names||F-box only protein 18, F-box DNA helicase 1, FBXO18, FBH1, FBX18|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||3'-5' DNA helicase and substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks (PubMed:11956208, PubMed:23393192). Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed:17724085, PubMed:19736316). Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress (PubMed:23319600, PubMed:23361013). Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal (PubMed:25772361). In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location (PubMed:25585578).|
|Cellular Location||Nucleus. Chromosome. Note=Accumulates at sites of DNA damage or replication stress (PubMed:19736316, PubMed:23677613) PCNA is required for localization to DNA damage sites (PubMed:23677613). Localizes to the nucleoplasm in absence of DNA damage (PubMed:23677613).|
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This gene encodes a member of the F-box protein family,members of which are characterized by an approximately 40 aminoacid motif, the F-box. The F-box proteins constitute one of thefour subunits of ubiquitin protein ligase complex called SCFs(SKP1-cullin-F-box), which function in phosphorylation-dependentubiquitination. The F-box proteins are divided into three classes:Fbws containing WD-40 domains, Fbls containing leucine-richrepeats, and Fbxs containing either different protein-proteininteraction modules or no recognizable motifs. The protein encodedby this gene belongs to the Fbx class. It contains an F-box motifand seven conserved helicase motifs, and has both DNA-dependentATPase and DNA unwinding activities. Alternatively splicedtranscript variants encoding distinct isoforms have been identifiedfor this gene.
Turnbull, C., et al. Nat. Genet. 42(6):504-507(2010)Fugger, K., et al. J. Cell Biol. 186(5):655-663(2009)Chiolo, I., et al. Mol. Cell. Biol. 27(21):7439-7450(2007)Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)Deloukas, P., et al. Nature 429(6990):375-381(2004)
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