|Other Names||Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, LTrpC-6, LTrpC6, Transient receptor potential p8, Trp-p8, TRPM8, LTRPC6, TRPP8|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration (PubMed:25559186). Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Membrane raft. Endoplasmic reticulum membrane. Note=Localizes to membrane rafts but is also located in the cell membrane outside of these regions where channel response to cold is enhanced compared to membrane rafts (By similarity). Located in the endoplasmic reticulum in prostate cancer cells.|
|Tissue Location||Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors.|
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Phosphatidylcholine (PC)-specific phospholipases D (PLDs;EC 18.104.22.168) catalyze the hydrolysis of PC to produce phosphatidicacid and choline. A range of agonists acting through Gprotein-coupled receptors and receptor tyrosine kinases stimulatethis hydrolysis. PC-specific PLD activity has been implicated innumerous cellular pathways, including signal transduction, membranetrafficking, and the regulation of mitosis (Hammond et al., 1995[PubMed 8530346]).
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Kang, D.W., et al. Cancer Res. 70(10):4233-4242(2010)Yin, H., et al. J. Biol. Chem. 285(18):13580-13588(2010)Kang, D.W., et al. PLoS ONE 5 (8), E12109 (2010) :
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