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RHEB Antibody (C-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q15382 |
|---|---|
| Clone Names | 71211161 |
| Gene ID | 6009 |
|---|---|
| Other Names | GTP-binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2 |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | RHEB {ECO:0000303|PubMed:8543055, ECO:0000312|HGNC:HGNC:10011} |
|---|---|
| Function | Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:12172553, PubMed:12906785, PubMed:12271141, PubMed:12842888, PubMed:12869586, PubMed:15340059, PubMed:15854902, PubMed:16098514, PubMed:20381137, PubMed:24529379, PubMed:22819219, PubMed:29416044, PubMed:32470140, PubMed:33157014). In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) (PubMed:33157014, PubMed:29236692). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes (PubMed:24529379). In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1 (PubMed:24529379, PubMed:33157014). Has low intrinsic GTPase activity (PubMed:15340059). |
| Cellular Location | Endomembrane system; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid- anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. Note=Farnesylation is required for recruitment to lysosomal membranes, where it activates the mTORC1 complex. |
| Tissue Location | Ubiquitous (PubMed:8543055). Highest levels observed in skeletal and cardiac muscle (PubMed:8543055) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
References
Yee, N.S., et al. Cancer Lett. 297(1):49-55(2010)Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Kuhn, F.J., et al. J. Biol. Chem. 285(35):26806-26814(2010)Gkika, D., et al. Oncogene 29(32):4611-4616(2010)Van Haute, C., et al. ScientificWorldJournal 10, 1597-1611 (2010) :
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