PRKAG1 Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P54619 |
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Clone Names | 101008282 |
Gene ID | 5571 |
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Other Names | 5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | PRKAG1 |
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Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:21680840, PubMed:24563466). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:21680840, PubMed:24563466). AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:21680840, PubMed:24563466). Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin (PubMed:21680840, PubMed:24563466). Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits (PubMed:21680840, PubMed:24563466). ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit (PubMed:21680840, PubMed:24563466). ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive (PubMed:21680840, PubMed:24563466). |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene belongs to thephosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles insignaling pathways involved in cell proliferation, oncogenictransformation, cell survival, cell migration, and intracellularprotein trafficking. This protein contains a lipid kinase catalyticdomain as well as a C-terminal C2 domain, a characteristic of classII PI3-kinases. C2 domains act as calcium-dependent phospholipidbinding motifs that mediate translocation of proteins to membranes,and may also mediate protein-protein interactions. The PI3-kinaseactivity of this protein is not sensitive to nanomolar levels ofthe inhibitor wortmanin. This protein was shown to be able to beactivated by insulin and may be involved in integrin-dependentsignaling.
References
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Kostakis, G.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109 (5), E53-E58 (2010) :Koutros, S., et al. Cancer Res. 70(6):2389-2396(2010)Ng, S.K., et al. Biochem. Biophys. Res. Commun. 387(2):310-315(2009)
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