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WT1 Antibody (Center E361) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P19544 |
|---|---|
| Clone Names | 80821105 |
| Gene ID | 7490 |
|---|---|
| Other Names | Wilms tumor protein, WT33, WT1 |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | WT1 |
|---|---|
| Function | Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921). |
| Cellular Location | Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190). Shuttles between nucleus and cytoplasm. {ECO:0000250, ECO:0000269|PubMed:15520190} [Isoform 4]: Nucleus, nucleoplasm |
| Tissue Location | Expressed in the kidney and a subset of hematopoietic cells |
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Background
This gene encodes a transcription factor that containsfour zinc-finger motifs at the C-terminus and aproline/glutamine-rich DNA-binding domain at the N-terminus. It hasan essential role in the normal development of the urogenitalsystem, and it is mutated in a small subset of patients with Wilm'stumors. This gene exhibits complex tissue-specific and polymorphicimprinting pattern, with biallelic, and monoallelic expression fromthe maternal and paternal alleles in different tissues. Multipletranscript variants have been described. In several variants, thereis evidence for the use of a non-AUG (CUG) translation initiationsite upstream of and in-frame with the first AUG. Authors ofPMID:7926762 also provide evidence that WT1 mRNA undergoes RNAediting in human and rat, and that this process istissue-restricted and developmentally regulated. [provided byRefSeq].
References
Sitaram, R.T., et al. Br. J. Cancer 103(8):1255-1262(2010)Dohi, S., et al. Anticancer Res. 30(8):3187-3192(2010)Rocquain, J., et al. BMC Cancer 10, 401 (2010) :Wagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) :Mitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)
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