|Other Names||Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35, CDK8|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of thecyclin-dependent protein kinase (CDK) family. CDK family membersare highly similar to the gene products of Saccharomyces cerevisiaecdc28, and Schizosaccharomyces pombe cdc2, and are known to beimportant regulators of cell cycle progression. This kinase and itsregulatory subunit cyclin C are components of the RNA polymerase IIholoenzyme complex, which phosphorylates the carboxy-terminaldomain (CTD) of the largest subunit of RNA polymerase II. Thiskinase has also been shown to regulate transcription by targetingthe CDK7/cyclin H subunits of the general transcription initiationfactor IIH (TFIIH), thus providing a link between the'Mediator-like' protein complexes and the basal transcriptionmachinery.
Seo, J.O., et al. Oncol. Rep. 24(1):285-291(2010)Tsutsui, T., et al. Seikagaku 82(3):191-199(2010)Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010)Donner, A.J., et al. Nat. Struct. Mol. Biol. 17(2):194-201(2010)Alarcon, C., et al. Cell 139(4):757-769(2009)
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