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WRN Antibody (Center T802) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q14191 |
|---|---|
| Clone Names | 80612087 |
| Gene ID | 7486 |
|---|---|
| Other Names | Werner syndrome ATP-dependent helicase, DNA helicase, RecQ-like type 3, RecQ3, Exonuclease WRN, 31--, RecQ protein-like 2, WRN, RECQ3, RECQL2 |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | WRN |
|---|---|
| Synonyms | RECQ3, RECQL2 |
| Function | Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed:9611231, PubMed:9224595, PubMed:9288107). Also has 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang (PubMed:11863428). Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA (PubMed:11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding- protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed:9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed:9288107, PubMed:9224595, PubMed:9611231). Depletion leads to chromosomal breaks and genome instability (PubMed:33199508). |
| Cellular Location | Nucleus, nucleolus. Nucleus. Nucleus, nucleoplasm. Chromosome. Note=Gamma-irradiation leads to its translocation from nucleoli to nucleoplasm and PML regulates the irradiation-induced WRN relocation (PubMed:21639834). Localizes to DNA damage sites (PubMed:27063109). |
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Background
This gene encodes a member of the RecQ subfamily and theDEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNAhelicases are involved in many aspects of DNA metabolism, includingtranscription, replication, recombination, and repair. This proteincontains a nuclear localization signal in the C-terminus and showsa predominant nucleolar localization. It possesses an intrinsic 3'to 5' DNA helicase activity, and is also a 3' to 5' exonuclease.Based on interactions between this protein and Ku70/80 heterodimerin DNA end processing, this protein may be involved in the repairof double strand DNA breaks. Defects in this gene are the cause ofWerner syndrome, an autosomal recessive disorder characterized bypremature aging.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Perry, J.J., et al. J. Biol. Chem. 285(33):25699-25707(2010)Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)Kobayashi, J., et al. Mech. Ageing Dev. 131(6):436-444(2010)Ehrenberg, M., et al. Mol. Vis. 16, 1771-1775 (2010) :
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