|Other Names||Histone lysine demethylase PHF8, PHD finger protein 8, PHF8, KIAA1111, ZNF422|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Note=Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis|
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The protein encoded by this gene is a histone lysinedemethylase that preferentially acts on histones in the monomethylor dimethyl states. The encoded protein requires Fe(2+) ion,2-oxoglutarate, and oxygen for its catalytic activity. Defects inthis gene are a cause of mental retardation syndromic X-linkedSiderius type (MRXSSD). Four transcript variants encoding differentisoforms have been found for this gene.
Liu, W., et al. Nature 466(7305):508-512(2010)Qi, H.H., et al. Nature 466(7305):503-507(2010)Feng, W., et al. Nat. Struct. Mol. Biol. 17(4):445-450(2010)Yue, W.W., et al. FEBS Lett. 584(4):825-830(2010)Yu, L., et al. Cell Res. 20(2):166-173(2010)
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