|Other Names||Mitogen-activated protein kinase kinase kinase kinase 2, B lymphocyte serine/threonine-protein kinase, Germinal center kinase, GC kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, MEKKK 2, Rab8-interacting protein, MAP4K2, GCK, RAB8IP|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.|
|Cellular Location||Cytoplasm. Basolateral cell membrane; Peripheral membrane protein Golgi apparatus membrane; Peripheral membrane protein|
|Tissue Location||Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined|
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The protein encoded by this gene is a member of theserine/threonine protein kinase family. Although this kinase isfound in many tissues, its expression in lymphoid follicles isrestricted to the cells of germinal centre, where it mayparticipate in B-cell differentiation. This kinase can be activatedby TNF-alpha, and has been shown to specifically activate MAPkinases. This kinase is also found to interact with TNFreceptor-associated factor 2 (TRAF2), which is involved in theactivation of MAP3K1/MEKK1.
Bailey, S.D., et al. Diabetes Care (2010) In press :Fidalgo, M., et al. J. Cell. Sci. 123 (PT 8), 1274-1284 (2010) :Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)Wissing, J., et al. Mol. Cell Proteomics 6(3):537-547(2007)Chadee, D.N., et al. Mol. Cell. Biol. 22(3):737-749(2002)
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