|Other Names||DNA-directed RNA polymerase I subunit RPA2, RNA polymerase I subunit 2, DNA-directed RNA polymerase I 135 kDa polypeptide, RPA135, POLR1B|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).|
|Cellular Location||Nucleus, nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
Eukaryotic RNA polymerase I (pol I) is responsible for thetranscription of ribosomal RNA (rRNA) genes and production of rRNA,the primary component of ribosomes. Pol I is a multisubunit enzymecomposed of 6 to 14 polypeptides, depending on the species. Most ofthe mass of the pol I complex derives from the 2 largest subunits,Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither andGrummt, 1996 [PubMed 8921381]).
Wen, L., et al. Biochem. Biophys. Res. Commun. 367(4):846-851(2008)Wu, C., et al. Proteomics 7(11):1775-1785(2007)Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006)Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
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