|Other Names||Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SF3b130, STAF130, Spliceosome-associated protein 130, SAP 130, SF3B3, KIAA0017, SAP130|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.|
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This gene encodes subunit 3 of the splicing factor 3bprotein complex. Splicing factor 3b, together with splicing factor3a and a 12S RNA unit, forms the U2 small nuclearribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3acomplex binds pre-mRNA upstream of the intron's branch site in asequence independent manner and may anchor the U2 snRNP to thepre-mRNA. Splicing factor 3b is also a component of the minorU12-type spliceosome. Subunit 3 has also been identified as acomponent of the STAGA (SPT3-TAF(II)31-GCN5L acetylase)transcription coactivator-HAT (histone acetyltransferase) complex,and the TFTC (TATA-binding-protein-free TAF(II)-containingcomplex). These complexes may function in chromatin modification,transcription, splicing, and DNA repair.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Will, C.L., et al. RNA 10(6):929-941(2004)Cavusoglu, N., et al. Proteomics 3(2):217-223(2003)Will, C.L., et al. EMBO J. 21(18):4978-4988(2002)Martinez, E., et al. Mol. Cell. Biol. 21(20):6782-6795(2001)
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