|Other Names||GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1, O-FucT-1, POFUT1, FUT12, KIAA0180|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).|
|Cellular Location||Endoplasmic reticulum.|
|Tissue Location||Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas|
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This gene encodes a member of the glycosyltransferaseO-Fuc family. This enzyme adds O-fucose through an O-glycosidiclinkage to conserved serine or threonine residues in the epidermalgrowth factor-like repeats of a number of cell surface and secretedproteins. O-fucose glycans are involved in ligand-induced receptorsignaling. Alternative splicing of this gene results in twotranscript variants encoding different isoforms. [provided byRefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Stahl, M., et al. J. Biol. Chem. 283(20):13638-13651(2008)Luo, Y., et al. J. Biol. Chem. 280(12):11289-11294(2005)Shi, S., et al. Proc. Natl. Acad. Sci. U.S.A. 100(9):5234-5239(2003)Panin, V.M., et al. J. Biol. Chem. 277(33):29945-29952(2002)
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