TRUB1 Antibody (Center) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q8WWH5 |
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Clone Names | 91201302 |
Gene ID | 142940 |
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Other Names | Probable tRNA pseudouridine synthase 1, 5499-, TRUB1, PUS4 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | TRUB1 {ECO:0000303|PubMed:12736709, ECO:0000312|HGNC:HGNC:16060} |
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Function | Pseudouridine synthase that catalyzes pseudouridylation of mRNAs and tRNAs (PubMed:28073919, PubMed:31477916, PubMed:32926445). Mediates pseudouridylation of mRNAs with the consensus sequence 5'- GUUCNANNC-3', harboring a stem-loop structure (PubMed:28073919, PubMed:31477916). Constitutes the major pseudouridine synthase acting on mRNAs (PubMed:28073919). Also catalyzes pseudouridylation of some tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the psi GC loop of a subset of tRNAs (PubMed:32926445, PubMed:33023933). Promotes the processing of pri-let-7 microRNAs (pri-miRNAs) independently of its RNA pseudouridylate synthase activity (PubMed:32926445). Acts by binding to the stem-loop structure on pri- let-7, preventing LIN28-binding (LIN28A and/or LIN28B), thereby enhancing the interaction between pri-let-7 and the microprocessor DGCR8, which mediates miRNA maturation (PubMed:32926445). |
Cellular Location | Nucleus. Cytoplasm, cytosol. Note=Catalyzes pseudouridylation of mRNAs in the nucleus. |
Tissue Location | Highly expressed in heart, skeletal muscle and liver. Expressed at lower levels in lung, small intestine, kidney and spleen. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Pseudouridine is an abundant component of rRNAs and tRNAsand is enzymatically generated by isomerization of uridine bypseudouridine synthase (Zucchini et al., 2003 [PubMed12736709]).
References
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)Deloukas, P., et al. Nature 429(6990):375-381(2004)Zucchini, C., et al. Int. J. Mol. Med. 11(6):697-704(2003)
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