|Other Names||Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1301a was selected from the region of human Bak BH3 Domain. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||BAK, BCL2L7, CDN1|
|Function||In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.|
|Cellular Location||Mitochondrion membrane; Single-pass membrane protein|
|Tissue Location||Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Cartron, P.F., et al., Mol. Cell. Biol. 23(13):4701-4712 (2003).Mikhailov, V., et al., J. Biol. Chem. 278(7):5367-5376 (2003).Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002).Bellosillo, B., et al., Blood 100(5):1810-1816 (2002).Grutkoski, P.S., et al., Shock 17(1):47-54 (2002).
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