|Other Names||Protein Hook homolog 1, h-hook1, hHK1, HOOK1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13130c was selected from the Center region of HOOK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).|
|Cellular Location||Cytoplasm, cytoskeleton. Cytoplasm. Note=Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.|
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This gene encodes a member of the hook family ofcoiled-coil proteins, which bind to microtubules and organellesthrough their N- and C-terminal domains, respectively. The encodedprotein localizes to discrete punctuate subcellular structures, andinteracts with several members of the Rab GTPase family involved inendocytosis. It is thought to link endocytic membrane traffickingto the microtubule cytoskeleton. Several alternatively splicedtranscript variants have been identified, but the full-lengthnature of some of these variants has not been determined. [providedby RefSeq].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Xu, L., et al. Mol. Biol. Cell 19(12):5059-5071(2008)Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)Simpson, F., et al. Traffic 6(6):442-458(2005)
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