|Other Names||LIM and calponin homology domains-containing protein 1, LIMCH1, KIAA1102|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13141b was selected from the C-term region of LIMCH1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.|
|Cellular Location||Cytoplasm, cytoskeleton. Note=Associates with contractile actin stress fibers.|
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Provided below are standard protocols that you may find useful for product applications.
LIMCH1 contains one CH (calponin-homology) domain and one LIM zinc-binding domain. There are nine named isoforms.
Rose, J. Phd, et al. Mol. Med. (2010) In press :Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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