|Other Names||N-terminal Xaa-Pro-Lys N-methyltransferase 1, Alpha N-terminal protein methyltransferase 1A, Methyltransferase-like protein 11A, N-terminal RCC1 methyltransferase, X-Pro-Lys N-terminal protein methyltransferase 1A, NTM1A, N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processed, NTMT1, C9orf32, METTL11A, NRMT, NRMT1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13172a was selected from the N-term region of ME11A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||C9orf32, METTL11A, NRMT, NRMT1|
|Function||Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation (PubMed:24090352). Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.|
|Cellular Location||Nucleus. Note=Predominantly nuclear (PubMed:24090352)|
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Provided below are standard protocols that you may find useful for product applications.
The METTL11A gene encodes an N-terminal methyltransferasefor the RAN (MIM 601179) guanine nucleotide exchange factorregulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11Aenzyme alpha-N-methylates other protein targets such as SET (MIM600960) and RB (MIM 180200).
Tooley, C.E., et al. Nature 466(7310):1125-1128(2010)Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)Humphray, S.J., et al. Nature 429(6990):369-374(2004)Hu, R.M., et al. Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548(2000)
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