Puma BH3 Domain Antibody Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9BXH1 |
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Clone Names | 2041511 |
Gene ID | 27113 |
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Other Names | Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP1317a was selected from the region of human Puma BH3 Domain. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | BBC3 |
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Synonyms | PUMA |
Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity). |
Cellular Location | Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release |
Tissue Location | Ubiquitously expressed. |
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Provided below are standard protocols that you may find useful for product applications.
Background
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
References
Liu, F.T., et al., Biochem. Biophys. Res. Commun. 310(3):956-962 (2003).Hoque, M.O., et al., Cancer Lett. 199(1):75-81 (2003).Yu, J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(4):1931-1936 (2003).Han, J., et al., Proc. Natl. Acad. Sci. U.S.A. 98(20):11318-11323 (2001).Nakano, K., et al., Mol. Cell 7(3):683-694 (2001).
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